酵母β-葡聚糖检测试剂盒爱尔兰

酵母β-葡聚糖检测试剂盒 Yeast Beta Glucan Assay Kit 100次 爱尔兰 型号:JKY/K-YBGL

库号：M248783

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名称:酵母β-葡聚糖检测试剂盒

英文名: Yeast Beta Glucan Assay Kit

规格:100次

产地:爱尔兰

用于测量酵母中1,3:1,6 -β-葡聚糖和a-葡聚糖。

包装:5个玻璃小瓶和1个聚丙烯瓶。

参数:
在水中的溶解度:易溶
pH值:7.0
气味:无
形态:粉末和液体形式
保质期:冷冻5年或以上

成分:
名称 比例
外切-1 ,3-β-葡聚糖酶+β-葡萄糖苷酶 1瓶（玻璃）
淀粉葡萄糖苷酶酶溶液 1瓶（玻璃）
葡萄糖测定试剂 1瓶（玻璃）
葡萄糖试剂缓冲液 1瓶（聚丙烯）
葡萄糖标准溶液 1瓶（玻璃）
酵母β-葡聚糖控制粉 1瓶（玻璃）

为衡量1,3:1,6 - β -葡聚糖和A -葡聚糖在酵母中的筹备工作。
导言：
（ 1-3 ） - β -葡聚糖含量分布广泛的性质，特别是在藻类，
真菌和酵母，而且在高等植物。他们所服务的各种
生物学功能。它们构成了重大的结构性组成部分
细胞壁，他们作为存储碳水化合物和他们有时发挥
保护作用，形成在特定的网站针对特定
刺激如伤人。
1的重要意义（ 1-3 ） - β -葡聚糖的
葡萄酒业是有关葡萄酒中存在的特定
超高分子量（ 〜 800科威特第纳尔） β -葡聚糖，所产生的真菌，
灰霉病（灰腐病） 。这葡聚糖由一个（ 1-3 ） - β -链接
D -葡聚糖骨干的单D -葡萄糖基残留附
β - （ 1-6 ） ，以每三主链残留。的β -葡聚糖的通行证进入
的必须，随着酒精浓度上升后期葡萄酒
决策，聚集发生导致澄清和过滤
的问题。 β -葡聚糖的水平低10 mg / L时可造成严重
过滤problems2 。这些问题只能删除
除了特定的酶制剂活性多糖在此。
目前的检测方法这一β -葡聚糖在葡萄酒是
使用非特定的酒精沉淀试验。 β -葡聚糖含量也有
药用的影响及文献表明，有效的反
肿瘤性质的多糖成分提取一定的
株蘑菇，特别是灰树花也被称为
灰树花，可以归因于线性6支， 1,3 - β -葡聚糖。
3月5日它
一直声称，酵母β -葡聚糖的大幅度提高
功能的免疫系统激活的巨噬细胞，其中一个
主要部队的免疫系统。
这boolet描述的方法测量（ 1-3 ） （ 1-6 ） - β -
葡聚糖酵母和蘑菇。工作仍在继续的提取和
脱色程序分析β -葡聚糖的葡萄酒。
原则：
1,3:1,6 - β - D -葡聚糖， 1,3 - β -葡聚糖和α -葡聚糖是solubilised在
集中（ 37 ％ ; 10否）盐酸然后广泛
水解1.3 ñ盐酸在100 ℃ 2小时水解为D -葡萄糖
完成孵育的混合物高纯度外- 1 ,3 - β -
葡聚糖酶和β -葡萄糖苷酶。虽然一些β -葡聚糖可随时可溶性
在热水或热的氢氧化钾，这些溶剂的效果不理想的
solubilising的β -葡聚糖酵母或蘑菇。分析这些
葡聚糖需要事先部分酸水解删除凝胶形成
性能及共价键连接到其他多糖（如甲壳素）或
蛋白质

英文资料:

For the measurement of 1,3:1,6-ß-glucan and a-glucan in yeast preparations.

Catalogue Number: K-YBGL
Content: 100 assays per kit

Appearance Five glass vials and one polypropylene vial
Specific Gravity Not applicable
Solubility in Water Readily soluble
pH Value 7.0
Odour None
Form Powders and liquids
Stability Stable in a freezer for five or more years
Ingredients Name CAS Proportion
exo-1,3-b-Glucanase + b-Glucosidase enzymes 1 vial (glass)
Amyloglucosidase enzyme solution 1 vial (glass)
Glucose determination reagent 1 vial (glass)
Glucose reagent buffer 1 vial (polypropylene)
Glucose standard solution 1 vial (glass)
Yeast b-glucan control powder 1 vial (glass)

KITS:
Kits suitable for carrying out 100 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: exo-1,3-b-Glucanase (100 U/mL) plus b-Glucosidase
(20 U/mL) suspension, 2.0 mL.
Stable for > 4 years at 4°C.
Bottle 2: Amyloglucosidase (1630 U/mL) plus invertase
(500 U/mL) solution in 50 % v/v glycerol, 20 mL.
Stable for ~ 2 years at 4°C or > 4 years at -20°C.
Bottle 3: Glucose reagent buffer (concentrate; 50 mL).
Stable for ~ 2 years at 4°C or for > 4 years
at -20°C.
Bottle 4: Glucose determination reagent.
Reagent concentrations after dissolution in buffer:
Glucose oxidase > 12,000 U/litre.
Peroxidase > 650 U/litre.
4-Aminoantipyrine 0.4 mM.
Stable for > 4 years at -20°C.
Bottle 5: D-Glucose standard solution (5 mL, 1.00 mg/mL) in
0.2 % w/v benzoic acid.
Stable for > 4 years at room temperature.
Bottle 6: Control yeast b-glucan preparation (~ 2 g, b-glucan
content stated on the bottle label).
Stable for > 5 years at room temperature.
PREPARATION OF REAGENT SOLUTIONS/SUSPENSIONS:
1. Add 8 mL of 200 mM sodium acetate buffer (pH 5.0) to
bottle 1 (i.e. dilute the contents of the vial to 10 mL).
Divide into appropriately sized aliquots and store in
polypropylene tubes at -20°C between use and on ice during
use. Once diluted, the reagent is stable for > 2 years at -20°C.
2. Use the contents of bottle 2 as supplied.
Stable for ~ 2 years at 4°C or > 4 years at -20°C.
3. Dilute the contents of bottle 3 to 1.0 L with distilled or
deionised water. Stable for > 2 years at 4°C.
4. Dissolve the contents of bottle 4 in the diluted contents of
bottle 3 (see the preceding reagent). Divide this reagent
mixture (GOPOD reagent) into aliquots of desired volume for
storage. Stable for 2-3 months at 4°C in a dark bottle,
or for > 12 months at -20°C.
2
5. Use the contents of bottle 5 as supplied.
Stable for > 4 years at room temperature.
6. Use the contents of bottle 6 as supplied.
Stable for > 5 years at room temperature.
REQUIRED REAGENTS (not supplied):
1. Sodium acetate buffer (200 mM, pH 5.0).
Add 11.6 mL of glacial acetic acid (1.05 g/mL) to 900 mL of
distilled water and adjust to pH 5.0 using 4 M (16 g/100 mL)
sodium hydroxide solution. Adjust the volume to 1 litre.
Stable for ~ 1 year at 4°C.
2. Sodium acetate buffer (1.2 M, pH 3.8).
Add 69.6 mL of glacial acetic acid (1.05 g/mL) to 800 mL of
distilled water and adjust to pH 3.8 using 4 M sodium
hydroxide. Adjust the volume to 1 litre with distilled water.
Stable for > 2 years at room temperature.
3. Potassium Hydroxide (2 M).
Add 112 g of KOH to 800 mL of distilled water and dissolve
by stirring. Adjust the volume to 1 litre.
Stable for > 2 years at room temperature.
4. Hydrochloric acid (37 % v/v; ~10 M).
Merck No. 100317-2500.
Stable for > 10 years at room temperature.
EQUIPMENT (RECOMMENDED):
1. Glass test tubes (round bottomed, 16 x 100 mm, 14 mL capacity).
2. Corning Culture Tubes. - Screw cap tubes , 20 x 125 mm (Fisher
Scientific Cat No. FB59563) plus caps (Cat. No. FB51355).
Screw cap tubes, 16 x 125 mm (Fisher Scientific Cat. No.TKV-
173-030B) plus caps (Cat. No.TKV-178-020V).
Fisher Scientific, interact@fisher.co.uk
3. Boiling water bath (deep-fry cooker filled with water).
4. Micro-pipettors, 100 μlitre (e.g. Gilson Pipetman® or Rainin
EDP-2® motorised dispenser).
5. Positive displacement pipettor e.g. Eppendorf Multipette®
- with 5.0 mL Combitip® (to dispense 0.1 mL aliquots of
hydrolysed sample solution and 0.2 mL of enzymes).
- with 25 mL Combitip® (to dispense 1.5 mL aliquots of 37 %
HCl and 3.0 mL of GOPOD Reagent). .
6. Magnetic stirrer plus stirrer bars (5 x 15 mm).
7. Analytical balance.
3
8. Spectrophotometer set at 510 nm.
9. Vortex mixer.
10. Thermostatted water bath set at 40.0°C.
11. Bench centrifuge (required speed 3,000 rpm; i.e. approx.1,500g),
with tube holders to accommodate 20 x 125 mm tubes.
4
NOTE:
The tube holders routinely supplied with bench centrifuges
accommodate tubes of up to 16 mm diameter. In the current
method, the optimal tube diameter is 20 mm (to accommodate a
sample size of 100 mg). If a tube holder which can hold 20 mm
diameter tubes is not available, then use Corning Culture Tubes,
16 x 125 mm, as described above. In this case, reduce the sample
size and all volumes to 50 % of that described in the method.
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用途:食品
分类:囯际直购